干擾素γ(IFNg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- 編號USEA049Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.1pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 977 ¥ 1395 ¥ 6278 ¥ 11858 ¥ 97650
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測干擾素γ(IFNg)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的干擾素γ(IFNg)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 93-102 | 98 |
| EDTA plasma(n=5) | 78-101 | 93 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的干擾素γ(IFNg)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中干擾素γ(IFNg)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 96-103% | 81-99% | 94-102% |
| EDTA plasma(n=5) | 78-96% | 83-94% | 88-103% | 79-97% |
| heparin plasma(n=5) | 91-99% | 97-105% | 80-90% | 94-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將干擾素γ(IFNg)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的干擾素γ(IFNg)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的干擾素γ(IFNg)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的干擾素γ(IFNg)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA049Ra62 | 干擾素γ(IFNg)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA049Ra01 | 干擾素γ(IFNg)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA049Ra61 | 干擾素γ(IFNg)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA049Ra02 | 干擾素γ(IFNg)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA049Ra61 | 干擾素γ(IFNg)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA049Ra01 | 干擾素γ(IFNg)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA049Ra01 | 干擾素γ(IFNg)多克隆抗體 | WB; IHC |
| UPAA049Ra02 | 干擾素γ(IFNg)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA049Ra06 | 干擾素γ(IFNg)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA049Ra81 | 干擾素γ(IFNg)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA049Ra71 | 干擾素γ(IFNg)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA049Ra22 | 干擾素γ(IFNg)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA049Ra23 | 干擾素γ(IFNg)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA049Ra21 | 干擾素γ(IFNg)單克隆抗體 | WB; IHC; ICC; IP. |
| UMEA049Ra | 干擾素γ(IFNg)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA049Ra | 干擾素γ(IFNg)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA049Ra | 干擾素γ(IFNg)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA049Ra | 干擾素γ(IFNg)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA049Ra01 | 干擾素γ(IFNg)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UCGA049Ra01 | 干擾素γ(IFNg)cDNA | PCR、蛋白表達等 |
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| Experimental Cell Research | CD4+ CD25+ Tregs as dependent factor in the course of bleomycin-induced pulmonary fibrosis in mice [Pubmed: 31678213] |
| International Immunopharmacology | Melatonin alleviates oxidative stress in sleep deprived mice: Involvement of small intestinal mucosa injury [Pubmed: 31835081] |
| International Journal of Rheumatic Diseases | Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis [Pubmed: 31840939] |
| Frontiers in Immunology | The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the?… [Pubmed: 31921131] |
| Nutrients | Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes Rats [Pubmed: 31887996] |
| Transboundary and Emerging Diseases | Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856] |
| Nanomedicine | The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999] |
| Scientific Reports | Serum sHLA-G: Significant diagnostic biomarker with respect to therapy and immunosuppressive mediators in Head and Neck Squamous Cell Carcinoma [Pubmed: 32123232] |
| American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
| Journal of Inflammation Research | Active Cousinia thomsonii Extracts Modulate Expression of Crucial Proinflammatory Mediators/Cytokines and NFκB Cascade in Lipopolysaccharide-Induced?… [Pubmed: 33173324] |
| Toxicol Appl Pharmacol | Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling [Pubmed: 33171191] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT [Pubmed: 33083950] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| DRUG AND CHEMICAL TOXICOLOGY | Toxicological impact of sodium benzoate on inflammatory cytokines, oxidative stress and biochemical markers in male Wistar rats [Pubmed: 33003960] |
| Life Sci | PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102] |
| Vet Res | Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap (d41) protein and analysis of its immunogenicity in mice and swine [Pubmed: 32907618] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| EXPERIMENTAL DERMATOLOGY | Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins [Pubmed: 32682346] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?… [Pubmed: 32680553] |
| Life Sci | Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140] |
| cancer immunology immunotherapy | TIM-3 blockade combined with bispecific antibody MT110 enhances the anti-tumor effect of γδ T cells [Pubmed: 32588076] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| Molecular Medicine Reports | Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| BIOTECHNOLOGY LETTERS | Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway [Pubmed: 32567013] |
| Int Immunopharmacol | Celastrol attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [Pubmed: 32402948] |
| Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra [] | |
| Journal of Functional Foods | Polyphenolic extracts from Wushan tea leaves attenuate hepatic injury in CCl4-treated mice [] |
| Indian Journal of Animal Research | Immunological Effects of a Single Dose of PLGA Nanoparticles Encapsulated Peptide in Broilers in Comparison to Traditional Vaccines against Infectious Bursal … [] |
| Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period [] | |
| Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine [] | |
| Scientific Reports | Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model [33239756] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| Eur Rev Med Pharmacol Sci | Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963] |
| Biomed Pharmacother | Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model [33765583] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Life Sciences | p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis [33915129] |
| Infect Drug Resist | Talaromyces marneffei and Burkholderia cepacia Co-Infection in a HIV-Uninfected Patient with Anti-Interferon-|? Autoantibodies [34140785] |
| Vaccines | Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious??- [33670249] |
| Pharmaceutics | PK/PD Modeling of the PDE7 Inhibitor?aGRMS-55 in a Mouse Model of Autoimmune Hepatitis. Pharmaceutics 2021, 13, 597 [33919375] |
| Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR|á/NF-|êB signaling pathways [34162146] | |
| How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? [] | |
| Amplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ?°Combo?± Nanoagent Together With PD-L1 Blockade In??- [] | |
| World J Tradit Chin Med | Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement [] |
| Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094] |
| J Pharm Pharmacol | Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress [34244746] |
| J Nanobiotechnology | Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo” nanoagent together with PD-L1 blockade in?… [34225744] |
| Neoplasia | PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633] |
| Sci Rep | Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology [34508139] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?… [34802039] |
| Infect Drug Resist | Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions [34785914] |
| Environmental Research | In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and?… [34863982] |
| Eur J Pharm Sci | Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment [34314841] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704] |
| Poult Sci | Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent?… [Pubmed:35063807] |
| ACS Appl Mater Interfaces | Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform [Pubmed:35138103] |
| Theranostics | Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced?… [Pubmed:35401832] |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway [Pubmed:35366745] |
| Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131] |
| Infection and Drug Resistance | Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic?… [Pubmed:35592103] |
| Sci Rep | Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces?… [Pubmed:35641599] |
| Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356] |
| Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs [Pubmed:35215787] |
| Antioxidants | Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress [Pubmed:35204158] |
| Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis [Pubmed:35355860] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766] |
| researchsquare | MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus [] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows [] |
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