甘露糖結(jié)合凝集素1(LMAN1)多克隆抗體
Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1)
F5F8D; FMFD1; MCFD1; MR60; Gp58; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53
- 編號UPAC567Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPC567Hu01-甘露糖結(jié)合凝集素1(LMAN1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 315 ¥ 735 ¥ 1050 ¥ 2625 ¥ 10500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human LMAN1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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FITC staining on IF;<br/>Sample: Human Hela cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human LMAN1 Antibody<br/>Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
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Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: MCF7 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: LMAN1 knockout Hela cell lysate;
Predicted MW: 58kd
Observed MW: 58kd
Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human LMAN1 Polyclonal Antibody (Catalog PAC567Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對LMAN1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別LMAN1。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunofluorescence:5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPC567Hu01 | 甘露糖結(jié)合凝集素1(LMAN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC567Hu01 | 甘露糖結(jié)合凝集素1(LMAN1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
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